Somatic Embryogenesis and Plant Regeneration from Hypocotyl and Leaf Explants of Brassica Oleracea var. Botrytis (Cauliflower)

Journal title

Acta Biologica Cracoviensia s. Botanica




vol. 53


No 1



somatic embryogenesis ; cauliflower ; Brassica oleracea var. botrytis ; embryogenic callus ; tissue culture

Divisions of PAS

Nauki Biologiczne i Rolnicze


Biological Commission of the Polish Academy of Sciences – Cracow Branch




Artykuły / Articles


DOI: 10.2478/v10182-011-0004-5 ; ISSN 0001-5296 ; eISSN 1898-0295


Acta Biologica Cracoviensia s. Botanica; 2011; vol. 53; No 1


Ahmed A. (2011), Effect of picloram, additives and plant growth regulators on somatic embryogenesis of <i>Phyla nodiflora</i> (L.) Greene, Brazilian Archives of Biology and Technology, 54, 7, ; Ahloowalia B. (1991), Somatic embryos in monocots: The genesis and genetic stability, Revue de Cytolgie et de Biologie Vegetales, Le Botaniste, 14, 223. ; Ali A. (2007), Effect of different explants and media compositions for efficient somatic embryogenesis in sugarcane (<i>Saccharum officinarum</i>), Pakistan Journal of Botany, 39, 6, 1961. ; Arnold V. (2002), Developmental pathways of somatic embryogenesis, Plant Cell Tissue Organ Culture, 69, 233, ; Bhalla P. (1999), In vitro propagation of cauliflower, <i>Brassica oleracea</i> var. <i>botrytis</i> for hybrid seed production, Plant Cell Tissue and Organ Culture, 56, 89, ; Chikkala V. (2008), Regeneration from leaf explants and protoplasts of <i>Brassica oleracea</i> var. <i>botrytis</i> (cauliflower), Scientia Horticulturae, 119, 330, ; Duncan D. (1955), Multiple range and multiple F-tests, Biometrics, 11, 1, ; Eimert K. (1992), Transformation of cauliflower (<i>Brassica oleracea</i> var. <i>botrytis</i>)-an experimental survey, Plant Molecular Biology, 19, 485, ; Fransz P. (1994), Improvement of protoplast regeneration from a recalcitrant inbred line of <i>Brassica oleracea</i>: a morphogenetic analysis, Plant Science, 98, 87, ; Ikeda-Iwai M. (2003), Stress-induced somatic embryogenesis in vegetative tissues of, Arabidopsis thaliana. The Plant Journal, 34, 107. ; Jourdan P. (1990), Improved protoplast culture and stability of cytoplasmic traits in plants regenerated from leaf protoplasts of cauliflower (<i>Brassica oleracea</i> ssp. <i>botrytis</i>), Plant Cell Tissue and Organ Culture, 21, 227, ; Karami O. (2008), Induction of embryogenic callus and plant regeneration in Carnation (<i>Dianthus caryophyllus</i> L.), Online Journal of Biological Sciences, 8, 4, 68, ; Kieffer M. (1995), Rapid mass production of cauliflower propagules from fractionated and graded curd, Plant Science, 107, 229, ; Kieffer M. (2001), A cost effective protocol for in vitro mass propagation of cauliflower, Plant Science, 160, 1015, ; Kumar A. (1993), Rapid in vitro propagation of cauliflower, Plant Science, 90, 175, ; Leroy X. (2000), Cauliflower somatic embryogenesis and analysis of regenerant stability by ISSRs, Plant Cell Report, 19, 1102, ; Lv L. (2005), Study on transformation of cowpea trypsin inhibitor gene into cauliflower (<i>Brassica oleracea</i> var. <i>botrytis</i>), African Journal of Biotechnology, 4, 1, 45. ; Martin K. (2005), Direct and indirect somatic embryogenesis on cotyledon explants of <i>Quassia amara</i> L., an antileukaemic drug plant, In Vitro Cellular and Developmental Biology, 41, 54, ; Murashige T. (1962), A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiology Plantarum, 51, 473, ; Murthy H. (2008), Recurrent somatic embryogenesis and plant regeneration in <i>Coriandrum sativum</i> L, Scientia Horticulturae, 118, 168, ; Namasivayam P. (2007), Acquisition of embryogenic competence during somatic embryogenesis, Plant Cell, Tissue and Organ Culture, 90, 1, ; Pareek L. (1978), Somatic embryogenesis in leaf callus from cauliflower (<i>Brassica oleracea</i> var. <i>botrytis</i>), Plant Science Letters, 11, 311, ; Pogrebnyak N. (2006), Collard and cauliflower as a base for production of recombinant antigens, Plant Science, 171, 677, ; Schumann G. (1995), Biotechnology in Agriculture and Forestry, vol. 30: Somatic Embryogenesis and Synthetic Seed, 1, 71. ; Sharp W. (1980), The physiology of in vitro sexual embryogenesis, Horticulture Review, 2, 268. ; Qin Y. (2006), Shoot differentiation, regeneration and analysis of somaclonal variation by RAPD, Hereditas, 143, 91, ; Vatsya B. (1982), Plant regeneration from cotyledonary protoplasts of cauliflower (<i>Brassica oleracea</i> var. <i>botrytis</i>), Protoplasma, 113, 161, ; Vikrant A. (2001), Direct as well as indirect somatic embryogenesis from immature (unemerged) inflorescence of a minor millet <i>Paspalum scrobiculatum</i> L, Euphytica, 120, 167, ; Watts L. (1963), Investigations into the breeding system of cauliflower <i>Brassica oleracea</i> var. <i>botrytis</i> (L.), Euphytica, 12, 323. ; William E. (1986), Somatic embryogenesis: factors influencing coordinate behavior of cells as an embryogenic group, Annals of Botany, 57, 44. ; Yang Q. (1992), A study of factors affecting anther culture of cauliflower (<i>Brassica oleracea</i> var. <i>botrytis</i>), Plant Cell Tissue and Organ Culture, 28, 289, ; Zobayed S. (1999), Cauliflower shoot-culture: effects of different types of ventilation on growth and physiology, Plant Science, 141, 209,