The presence of lipopolysaccharide (LPS) in blood induces an
inflammatory response which leads to multiple organ dysfunction and
numerous metabolic disorders. Uncontrolled, improper or late
intervention may lead to tissue hypoxia, anaerobic glycolysis and a
disturbance in the acid -base balance. The effects of LPS-induced
toxemia on biological and immunological markers were well studied.
However, parameters such as base excess, ions, and acid-base balance
were not fully investigated. Therefore, the objective of this study was
to examine these blood parameters collectively in LPS-induced
inflammatory toxemia in rat’s model. After induction of toxemia by
injecting LPS at a rate of 5 mg/kg body weight intravenously, blood was
collected from the tail vein of twenty rats and immediately analyzed.
After 24 hours, the animals were sacrificed and the blood was collected
from the caudal vena cava. The results revealed that the levels of pH,
bicarbonate, partial pressure of oxygen, oxygen saturation, Alveolar
oxygen, hemoglobin, hematocrit, magnesium (Mg2+), and calcium (Ca2+)
were significantly decreased. On the other side, the levels of Base
excess blood, Base excess extracellular fluid, partial pressure of
carbon dioxide, lactate, Ca2+/Mg2+, potassium, and chloride were
significantly increased compared to those found pre toxemia induction.
However, sodium level showed no significant change. In conclusion, Acute
LPS-toxemia model disturbs acid-base balance, blood gases, and ions.
These parameters can be used to monitor human and animal toxemic
inflammatory response induced by bacterial LPS conditions to assist in
the management of the diagnosed cases.