@ARTICLE{Isah_Tasiu_IN_2013, author={Isah, Tasiu and Mujib, Abdul}, volume={vol. 55}, number={No 2}, journal={Acta Biologica Cracoviensia s. Botanica}, howpublished={online}, year={2013}, publisher={Biological Commission of the Polish Academy of Sciences – Cracow Branch}, abstract={An efficient system for plant regeneration of Senna occidentalis from hypocotyl-derived callus was developed. Callus was induced from leaf and hypocotyl explants on MS medium amended with 9.04 μM 2,4-D + 2.22 μM BAP and 10.74 μM NAA + 2.22 μM BAP. Medium browning due to leaching of compounds from callus was encountered and ameliorated through incorporation of 2.84 μM ascorbic acid. Leaf-derived callus showed no shoot induction ability, while hypocotyl-derived callus produced shoots in all cytokinin-amended treatments and also in combination with 2.68 μM NAA. For shoot formation, BAP-augmented treatments were better than medium with Kin added. Rhizogenesis was better on 1/2 MS basal medium with IBA than in the NAA and IAA treatments. Regenerated plants were acclimatized with 94% survival and showed similar morphology to field-grown plants.}, type={Artykuły / Articles}, title={IN VITRO PLANT REGENERATION OF COFFEE SENNA (SENNA OCCIDENTALIS) FROM HYPOCOTYL-DERIVED CALLUS}, URL={http://sp.czasopisma.pan.pl/Content/81806/PDF/13_isah.pdf}, doi={10.2478/abcsb-2013-0031}, keywords={Senna occidentalis, callus, regeneration. shoot induction, rhizogenesis, acclimatization}, }