@ARTICLE{Siong_Poon_Kok_Somatic_2011, author={Siong, Poon Kok and Taha, Rosna Mat and Rahiman, Fatimah Abdul}, volume={vol. 53}, number={No 1}, journal={Acta Biologica Cracoviensia s. Botanica}, howpublished={online}, year={2011}, publisher={Biological Commission of the Polish Academy of Sciences – Cracow Branch}, abstract={We investigated direct and indirect formation of somatic embryogenesis in Brassica oleracea var. botrytis (cauliflower), a very important vegetable crop worldwide. Direct somatic embryogenesis, which is rather rare, was achieved in culture of 2-week-old hypocotyl explants of Brassica oleracea var. botrytis on MS medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5; 1.0; and 1.5 mg/l kinetin. Initial induction of embryogenic callus was achieved on MS supplemented with very low concentrations of 2,4-D (0.05 mg/l and 0.1 mg/l). Indirect somatic embryogenesis from leaf sections was obtained on MS supplemented with 0.05 or 0.1 mg/l 2,4-D. We examined various stages of somatic embryos (globular, heart, torpedo, cotyledonary). More embryos per explant were produced through the indirect pathway (23-25) than through the direct pathway (14-19). The number of embryos produced was high. There is a potential for recurrent, repeated or secondary somatic embryogenesis, possibly an unlimited source for mass propagation and ideal for synthetic seed production in this species. Plant regeneration was achieved on half-strength MS medium without any hormones.}, type={Artykuły / Articles}, title={Somatic Embryogenesis and Plant Regeneration from Hypocotyl and Leaf Explants of Brassica Oleracea var. Botrytis (Cauliflower)}, URL={http://sp.czasopisma.pan.pl/Content/81717/PDF/abc53_1_04.pdf}, doi={10.2478/v10182-011-0004-5}, keywords={somatic embryogenesis, cauliflower, Brassica oleracea var. botrytis, embryogenic callus, tissue culture}, }